Journal: Neural Regeneration Research

Article Title: Protein hairy enhancer of split-1 expression during differentiation of muscle-derived stem cells into neuron-like cells ★

doi: 10.3969/j.issn.1673-5374.2012.028.003

Figure Lengend Snippet: Expression of hairy enhancer of split-1 (Hes1) protein (specific staining of muscle-derived stem cells) after differentiation in the experimental group and the control group (immunofluorescence cytochemistry staining, fluorescence microscopy, × 400). (A) In the control group, the red fluorescence (Hes1) was strong (arrows), and nuclei stained blue. (B) The cytoplasm of neuron-like cells was negative for Hes1. There was no Hes1 present in the experimental group and blue staining was very strong in the nucleus (arrows).

Article Snippet: Cells were then incubated in confining liquid for 20 minutes, followed by incubation with rabbit anti-rat Hes1 polyclonal primary antibody (1:100; Santa Cruz Biotechnology) at 4°C overnight.

Techniques: Expressing, Staining, Derivative Assay, Control, Immunofluorescence, Fluorescence, Microscopy

Journal: Neural Regeneration Research

Article Title: Protein hairy enhancer of split-1 expression during differentiation of muscle-derived stem cells into neuron-like cells ★

doi: 10.3969/j.issn.1673-5374.2012.028.003

Figure Lengend Snippet: Hes1 protein expression as detected by western blot assay during the differentiation of muscle-derived stem cells into neurons. (A) Western blot analysis showed that Hes1 was down-regulated in neuron-like cells induced after 6 days in culture. (B) Western blot semi-quantitative analysis results. The data (absorbance ratio to β-actin) are expressed as mean ± SD; the experiment was repeated five times. a P < 0.05, vs . control group using two samples t -test. 1: Experimental group; 2: control group.

Article Snippet: Cells were then incubated in confining liquid for 20 minutes, followed by incubation with rabbit anti-rat Hes1 polyclonal primary antibody (1:100; Santa Cruz Biotechnology) at 4°C overnight.

Techniques: Expressing, Western Blot, Derivative Assay, Control